A solution formulated to selectively disrupt erythrocytes while preserving other cell types, particularly leukocytes, is essential in many biological assays. This formulation enables researchers to isolate and analyze white blood cells or other components within a whole blood sample by selectively removing the predominant red blood cells. A typical preparation includes ammonium chloride, tris buffer, and EDTA, carefully balanced to maintain optimal pH and osmotic conditions for effective erythrocyte rupture and minimized damage to the cells of interest.
The use of such a solution streamlines downstream analyses, providing a clearer signal for subsequent cellular investigations. By removing the overwhelming presence of erythrocytes, researchers can more easily quantify and characterize the remaining cell population. This procedure has become a standard laboratory practice, significantly enhancing the accuracy and efficiency of immunological, hematological, and molecular biology experiments since its initial development and refinement over decades of scientific investigation. The application improves flow cytometry, cell culture, and DNA/RNA extraction protocols.
